How is bacterial DNA cross-linked to putative binding proteins for chromatin immunoprecipitation (ChIP)?

Question

Quizplus · Accepted Answer

Bacteria are treated with formaldehyde to cross-link DNA and putative binding proteins for ChIP. This creates covalent bonds between the proteins and the DNA, allowing for their isolation together. UV light can also be used for cross-linking, but it is less commonly used in bacterial ChIP experiments. Heat is not typically used for cross-linking in ChIP assays.

How Is Bacterial DNA Cross-Linked to Putative Binding Proteins for Chromatin