In the 3-step assay for determination of cholesterol concentration,cholesteryl esters must be cleaved by an enzyme.This hydrolytic cleavage forms:

Question

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Cholesteryl esters are cleaved by the enzyme cholesterol esterase to form cholesterol and fatty acids. This step is necessary for accurate measurement of cholesterol concentration in the 3-step assay. Option A is incorrect as it refers to the hydrolysis of triglycerides, which is not relevant to this particular assay. Option B refers to the breakdown of triglycerides into glycerol-1-phosphate and fatty acids, which is also not relevant to cholesterol measurement. Option D refers to the breakdown of cholesterol to cholestenone and hydrogen peroxide, which is not relevant to this particular assay.

In the 3-Step Assay for Determination of Cholesterol Concentration,cholesteryl Esters