In the 3-step assay for determination of cholesterol concentration,cholesteryl esters must be cleaved by which of the following enzymes for the initial step to occur?

Question

Quizplus · Accepted Answer

The cleavage of cholesteryl esters is a crucial initial step in the 3-step assay for cholesterol determination. The enzyme responsible for this cleavage is cholesteryl ester hydrolase, which breaks down the cholesteryl esters into cholesterol and fatty acids. This allows for the subsequent steps of the assay to measure the concentration of cholesterol accurately. Cholesterol oxidase, lipase, and peroxidase are not involved in this initial step.

In the 3-Step Assay for Determination of Cholesterol Concentration,cholesteryl Esters