In the Gram stain, the mordant is

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In the Gram stain, the mordant used is iodine. The iodine forms a complex with the crystal violet, trapping it inside the cell wall. This step is crucial for distinguishing between Gram-positive and Gram-negative bacteria. Gram-positive bacteria retain the crystal violet-iodine complex, appearing purple under the microscope, while Gram-negative bacteria are decolorized by the alcohol wash and counterstained with safranin, appearing pink under the microscope.

In the Gram Stain, the Mordant Is