Which of the following would interfere with the collection of a good sputum sample for Gram stain?
I.contamination of the sample with oral secretions
II.lack of productive cough
III.prior antibiotic therapy
IV.rinsing with mouthwash prior to sputum collection

Question

Quizplus · Accepted Answer

I. Contamination of the sample with oral secretions can interfere with the collection of a good sputum sample for Gram stain because it can introduce normal oral flora into the sample, which may not accurately represent the organisms present in the lower respiratory tract.
II. Lack of productive cough can make it difficult to obtain a sufficient amount of sputum for analysis.
III. Prior antibiotic therapy can alter the bacterial flora in the respiratory tract, making it difficult to identify the causative organism by Gram stain.
IV. Rinsing with mouthwash prior to sputum collection can also introduce normal oral flora into the sample and affect the accuracy of the Gram stain.

Which of the Following Would Interfere with the Collection of a Good