Which is TRUE regarding structure and function of RNAPII?
(I)Distortion of the DNA by ~90° isolates the template base such that it can be properly paired.
(II)The "clamp" moves over the DNA increasing processivity.
(III)RNAPII accomplishes proofreading by forcing mispaired nucleotides against the
"wall" causing distortion such that mispairs can be cleaved by the "rudder."
A)I,II,III
B)I,II
C)II,III
D)II only
E)III only

Question

Quizplus · Accepted Answer

(I) is not true as the distortion of the DNA by ~90° occurs in RNAP I, not RNAP II. (II) is true as the "clamp" of RNAPII moves over the DNA, increasing processivity. (III) is not true as RNAPII does not have proofreading activity. Instead, proofreading is accomplished by RNA polymerase II-associated factor 2 (RPA2) and other enzymes.

Which Is TRUE Regarding Structure and Function of RNAPII