In isoelectric focusing,a protein with a pH below the pI would
A) migrate toward the anode.
B) migrate toward the cathode.
C) stop migrating.
D) migrate, but there is no way to determine the direction.

Question

Quizplus · Accepted Answer

Isoelectric focusing separates proteins based on their isoelectric point (pI), the pH at which the protein has no net charge. Proteins with a pH below their pI will have a net positive charge and therefore migrate towards the negatively charged electrode (anode) in an electric field. Conversely, proteins with a pH above their pI will have a net negative charge and migrate towards the positively charged electrode (cathode). Therefore, a protein with a pH below its pI would migrate towards the anode.

In Isoelectric Focusing,a Protein with a PH Below the PI