Alteration of an amino acid at a site distant from the active site of an enzyme may alter the substrate specificity of the enzyme by
A) changing the optimum pH for the enzyme.
B) changing the intracellular location of the enzyme.
C) changing the binding site for an allosteric regulator.
D) changing the conformation of the enzyme.

Question

Quizplus · Accepted Answer

Altering the amino acid sequence of an enzyme can change its conformation and three-dimensional shape, which could affect the binding of substrates to the enzyme's active site. This change in the active site could also alter the substrate specificity of the enzyme, even if the altered amino acid is located far from the active site.

Alteration of an Amino Acid at a Site Distant from the Active