Yeast two-hybrid analysis functions due to which reason?
A) The DNA-binding domain and transcriptional activation domain of a separated transcription factor are only brought into close proximity when the two proteins of interest interact with each other.
B) The DNA-binding domain and the transcriptional activation domain of a separated transcription factor may remain stable independent of each other.
C) Yeast can be grown as either a haploid or a diploid cell.
D) All of the answers are correct.
E) None of the answers is correct.

Question

Quizplus · Accepted Answer

Yeast two-hybrid analysis is based on the principle that the DNA-binding domain and transcriptional activation domain of a transcription factor can function when brought into proximity by the interaction of two proteins of interest. These domains can remain stable independently, and the versatility of yeast genetics, including its ability to grow as haploid or diploid, facilitates this analysis.

Yeast Two-Hybrid Analysis Functions Due to Which Reason