Genetics: Analysis and Principles 5th Edition by Robert Brooker

تمرين 51

A research group has sequenced the cDNA and genomic DNA from a particular gene. The cDNA is derived from mRNA, so it does not contain introns. Here are the DNA sequences.cDNA:
5 -ATTGCATCCAGCGTATACTATCTCGGGCCCAATTAATGCCA-GCGGCCAGACTATCACCCAACTCGGTTACCTACTAGTATATC-CCATATACTAGCATATATTTTACCCATAATTTGTGTGTGGGTATA-CAGTATAATCATATA-3
Genomic DNA (contains one intron):
5 -ATTGCATCCAGCGTATACTATCTCGGGCCCAATTAATGCCAGCGGCCAGACTATCACCCAACTCGGCCCACCCCCCAGGTTTA-CACAGTCATACCATACATACAAAAATCGCAGTTACTTATCCCA-AAAAAACCTAGATACCCCACATACTATTAACTCTTTCTTTCTAG-GTTACCTACTAGTATATCCCATATACTAGCATATATTTTAC-CCATAATTTGTGTGTGGGTATACAGTATAATCATATA-3
Indicate where the intron is located. Does the intron contain the normal consensus splice site sequences based on those described in Figure 12.21 Underline the splice site sequences, and indicate whether or not they fit the consensus sequence.FIGURE 12.21 Consensus sequences for pre-mRNA splicing in complex eukaryotes. Consensus sequences exist at the intronexon boundaries and at a branch site found within the intron itself. The adenine nucleotide shown in blue in this figure corresponds to the adenine nucleotide at the branch site in Figure 12.22. The nucleotides shown in bold are highly conserved. Designations: A/C = A or C, Pu = purine, Py = pyrimidine, N = any of the four bases.
a.
FIGURE 12.22 Splicing of pre-mRNA via a spliceosome.