
Biochemistry 4th Edition by Reginald Garrett, Charles Grisham
النسخة 4الرقم المعياري الدولي: 9780495109358
Biochemistry 4th Edition by Reginald Garrett, Charles Grisham
النسخة 4الرقم المعياري الدولي: 9780495109358 تمرين 8
Restriction endonuclease mapping of a DNA fragment
A 10-kb DNA fragment digested with restriction endonuclease Eco RI yielded fragments 4 kb and 6 kb in size. When digested with Bam HI, fragments 1, 3.5, and 5.5 kb were generated. Concomitant digestion with both Eco RI and Bam HI yielded fragments 0.5, 1, 3, and 5.5 kb in size. Give a possible restriction map for the original fragment.
A 10-kb DNA fragment digested with restriction endonuclease Eco RI yielded fragments 4 kb and 6 kb in size. When digested with Bam HI, fragments 1, 3.5, and 5.5 kb were generated. Concomitant digestion with both Eco RI and Bam HI yielded fragments 0.5, 1, 3, and 5.5 kb in size. Give a possible restriction map for the original fragment.
التوضيح
Digestion with Eco RI gives two possible...
Biochemistry 4th Edition by Reginald Garrett, Charles Grisham
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