Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

تمرين 14

Design of a reporter gene construct to study promoter function
In your experiment in problem 12, you discover a gene that is strongly expressed in anaerobically grown yeast but turned off in aerobically grown yeast. You name this gene nox (for "no oxygen"). You have the "bright idea" that you can engineer a yeast strain that senses O₂ levels if you can isolate the nox promoter. Describe how you might make a reporter gene construct using the nox promoter and how the yeast strain bearing this reporter gene construct might be an effective oxygen sensor.