Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill

تمرين 2

When pure reduced cytochrome c is added to carefully prepared mitochondria along with ADP, P i , antimycin A, and oxygen, the cytochrome c becomes oxidized, and ATP is formed, with a P/O ratio approaching 1.0.
(a) Indicate the probable flow of electrons in this system.
(b) Why was antimycin A added
(c) What does this experiment tell you about the location of coupling sites for oxidative phosphorylation
(d) Write a balanced equation for the overall reaction (including cyt c oxidation and ATP synthesis).
(e) Calculate AG 0 , for the above reaction, using values from Table 15.1 and a G ° value for ATP hydrolysis of 30.5 kJ/mol.