Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill

تمرين 3

The following diagram shows one-half of a restriction site.
(a) Draw the other half.
(b) Use heavy arrows ( ) to identify type II cleavage sites that would yield blunt-ended duplex DNA products.
(c) Use light arrows ( ) to identify type II cleavage sites yielding staggered cuts that could be converted directly to recombinant DNA molecules by DNA ligase, with no other enzymes involved.
(d) If this were the recognition site for a type I restriction endonu-clease, where would cutting of the duplex occur
(e) If DNA sequences were completely random, how large an interval (in kilobase pairs) would you expect between identical copies of this sequence in DNA