Molecular Biology 5th Edition by Robert Weaver

تمرين 2

You are cloning the genome of a new DNA virus into pUC18. You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony. When you check the size of the inserts in each plasmid (blue and white), you are surprised to find that the plasmid from the blue colony contains a very small insert of approximately 60 bp, while the plasmid from the white colony does not appear to contain any insert at all. Explain these results.