Molecular Biology 5th Edition by Robert Weaver

تمرين 4

You incubate cells with an inhibitor of the protein kinase activity of TFIIH and then perform in vitro transcription and DNase footprinting experiments. What step in transcription would you expect to see blocked What kind of assay would reveal such a blockage Would you still expect to see a footprint at the promoter Why or why not If so, how large would the footprint be, compared to the footprint in the absence of the inhibitor