Molecular Biology 5th Edition by Robert Weaver

تمرين 3

You are investigating a tRNA Phe whose charging specificity appears to be affected by a C11-G24 base pair in the D stem. Design two experiments to show that changing this base pair changes the charging specificity of the tRNA. The first experiment should be a biochemical one using an in vitro reaction. The second should be a genetic one performed in vivo.