iGenetics 3rd Edition by Peter Russell

تمرين 6

Each of the following templates is added to an in vitro DNA synthesis reaction using the enzyme system isolated by Kornberg with 5
ATG-3? as a primer.
In what ways besides their sequence will the synthesized molecules differ if a trace amount of each of the following nucleotides is added to the reaction?
a.
32 P-dATP (dATP where the phosphorus closest to the 5
carbon is radioactive)b. 32 P-dAMP (dAMP where the phosphorus is radioactive)c.
32 P-dATP (dATP where the phosphorus furthest from the 5
carbon is radioactive)