Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

تمرين 8

Turnover Number. Carbonic anhydrase catalyzes the reversible hydration of carbon dioxide to form bicarbonate ion:
(1)
As we will discover in Chapter 8, this reaction is important in the transport of carbon dioxide from body tissues to the lungs by red blood cells. Carbonic anhydrase has a molecular weight of 30,000 and a turnover number (k cat value)of 1 × 10 6 sec -1. Assume that you are given 1 mLof a solution containing 2.0 µ g of pure carbonic anhydrase.
(a)At what rate (in millimoles of CO 2 consumed per second)will you expect this reaction to proceed under optimal conditions
(b)Assuming standard temperature and pressure, how much CO 2 is that in mL per second