Essential Genetics 6th Edition by Daniel Hartl

تمرين 7

A circular plasmid of 8 kb is digested with Eco RI (E) and/or Bam HI (B), and the digests are run on an agarose gel and stained. The results are shown below; molecular size standards are shown.

10.13 The Hessian fly Mayetiola destructor has among the
smallest genomes in insects, with a haploid genome size of
about 88 Mb. If this genome is digested with NotI (an eightbase
cutter), approximately how many DNA fragments would
be produced? Assume equal and random frequencies of the four
nucleotides.
10.14 A circular plasmid of 8 kb is digested with Eco RI (E)
and/or Bam HI (B), and the digests are run on an agarose gel and
stained. The results are shown below; molecular size standards
are shown.