Essential Genetics 6th Edition by Daniel Hartl

تمرين 10

Mutagenesis of a RAS gene of budding yeast yields a temperature-sensitive conditional mutation.
(a) Would you expect a cell that is carrying a mutation that prevents Ras from exchanging GDP for GTP at the restrictive temperature to continue to divide at this temperature?
(b) Would the mutation be dominant or recessive?
(c) Would you expect a cell that is carrying a mutation that inactivates the GTPase activity of Ras at 36°C to continue to divide at the restrictive temperature?
(d) Would this mutation be dominant or recessive?