Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

تمرين 8

The restriction endonuclease, XhoI, recognizes the sequence 5?-CTCGAG-3? and cleaves between the C and T on each strand.
A. What is the calculated frequency of this sequence occurring in a genome?
B. The restriction endonuclease, SalI, recognizes the sequence 5?-GTCGAC-3? and cleaves between the G and T on each strand. Do you think the sticky ends produced after XhoI and SalI cleavage could adhere to each other? Explain your choice.