Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

تمرين 4

You want to study the potential interaction between nucleosome-bound DNA and a specific histone deacetylase. You decide to perform an electrophoretic mobility shift assay (EMSA). For a review of this technique, see Chapter 7. You use a 32 P end-labeled, linear template DNA that contains two nucle-osome positioning sites. You assemble two nucleosomes on the DNA template before incubation with and without the histone deacetylase. For some reactions, you use unmodified nucleo-somes. For other reactions, you use nucleosomes that are methylated at lysine 36 of the histone protein H3.

A. Based on the data, propose a model for the interaction between the histone deacetylase and nucleosome-bound DNA.
B. What type of protein domain do you predict allows the his-tone deacetylase to interact with the nucleosomes?
Adapted from Huh et al. (2012. EMBO J. 31: 3564?3574).