Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

تمرين 5

Review the principles of the two-hybrid assay in Box 19-1. Where in the cell must the bait and prey interaction take place to observe activation of the reporter gene? Provide one potential drawback to the yeast two-hybrid assay with respect to this requirement. What other potential drawbacks can you imagine?