Introduction to Biotechnology 3rd Edition by William Thieman ,Michael Palladino

تمرين 7

Consider the following sequence of DNA: 5'-TTTATGGG TTGGCCCGGGTCATGATT- 3' 3'-AAATCCCAACCGGGCCCAGT ACTAA- 5'
a. Transcribe each of these sequences into mRNA. Which DNA sequence (top or bottom strand) produces a functional mRNA containing a start codon What is the amino acid sequence of the polypeptide produced from this mRNA
b. For the DNA strand producing a functional mRNA, number each base from left to right with the first base numbered "1." Insert a "T" between bases 10 and 11, representing a base insertion mutation. Transcribe an mRNA from this new strand and translate it into a protein. Compare the amino acid sequence of this protein to the one translated in (a). What happened Explain.