Molecular Biology Of The Cell 6th Edition by Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

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Hoechst 33342 is a membrane-permeant dye that fluoresces when it binds to DN a. When a population of cells is incubated briefly with Hoechst dye and then sorted in a flow cytometer, which measures the fluorescence of each cell, the cells display various levels of fluorescence as shown in Figure Q17-1.