Biomedical Engineering: Bridging Medicine and Technology 1st Edition by Veronique Tran,Mark Saltzman

تمرين 10

The standard curves shown in Figure 11.32 have the following equations:
a. Assume that you measure the OD of five unknown solutions that contain BSA and get the following readings: A280 = 0.35, 0.02, 0.58, 1.23, and 0.44. What are the concentrations of BSA in each of these solutions?
b. What would the concentration of antibody be for the same set of measurements?
c. Do you have confidence in all of the estimated concentrations that you have calculated? If not, which values are suspicious to you, and why?
d. How would you apply this method to a mixture that contained both BSA and Ab?