Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

تمرين 1

Consider the scale-up of a chromatography column for purification of a protein. A column of
40 cm length is used with a superficial velocity of 40 cm/h. The peak concentration of the tar-
get protein exits at a time of 100 min. The standard deviation of the peak is 14 min.
a. How long must you wait to collect 90% of the protein?
b. If the same column is used, but velocity is increased to 60 cm/h and external or Taylor
dispersion controls, what will be the value of

c. If the column is lengthened to 60 cm while the velocity is at 60 cm/h, how will
hange?
Will the peak be sharper or broader than at 40 cm/h with a 40-cm column?