
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
النسخة 2الرقم المعياري الدولي: 9780130819086
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
النسخة 2الرقم المعياري الدولي: 9780130819086 تمرين 2
Consider an industrial-scale batch fermentation. A 10,000 l fermenter with
cells/ml
is the desired scale-up operation. Inoculum for the large tank is brought through a series of
seed tanks and flasks, beginning with a single pure colony growing on an agar slant. Assume
that a colony
plasmid-containing cells) is picked and placed in a test tube with 1 ml of
medium. Calculate how many generations will be required to achieve the required cell den-
sity in the 10,000 l fermenter. What fraction of the total population will be plasmid-free cells

is the desired scale-up operation. Inoculum for the large tank is brought through a series of
seed tanks and flasks, beginning with a single pure colony growing on an agar slant. Assume
that a colony

medium. Calculate how many generations will be required to achieve the required cell den-
sity in the 10,000 l fermenter. What fraction of the total population will be plasmid-free cells

التوضيح
According to the given data:
In industri...
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
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