Deck 17: Recombination and Transposition at the Molecular

Question 1

Transposons that utilize an RNA intermediate for transposition are called .

A) Retro elements
B) Retroposons
C) Retrotransposons
D) All of the answers are correct

Answer 1

D

Question 2

An attachment site for bacteriophage l have which of the following characteristic(s)?

A) Specific proteins in the cell membrane
B) A unique set of phospholipids that identify the organelle of interest
C) A lack of a cell wall
D) A specific DNA sequence that is recognized by the integrase enzyme
E) None of the answers are correct

Answer 2

D

Question 3

Insertion sequences can be identified by which of the following?

A) Presence of an integrase enzyme
B) High level of mutation
C) Presence of inverted repeats at the end of the sequence
D) Presence of a reverse transcriptase enzyme
E) All of the answers are correct

Answer 3

C

Question 4

In the Perry-Wolff experiments with harlequin chromosomes, colcemid was applied to the cells following labeling with BrdU for what reason?

A) To enhance the rate of recombination between chromatids
B) To reduce the rate of recombination between chromatids
C) To separate the chromosomes
D) As a stain
E) To stop the cells from completing mitosis

Question 5

Bacteriophage l inserts into a bacterial chromosome using which of the following?

A) Nucleotide excision repair
B) Endonuclease activity
C) Site-specific recombination
D) DNA gap repair
E) None of the answers are correct

Question 6

Which of the following most likely accounts for the majority of the recombination events during both DNA repair and meiosis?

A) A nick in only one strand of the DNA
B) The double-stranded break model
C) DNA gap repair synthesis
D) Heteroduplex formation

Question 7

Which of the following is incorrect regarding immunoglobins?

A) They are produced by B cells
B) They recognize a specific epitope
C) The human genome contains a unique gene for each immunoglobin produced
D) They are all proteins

Question 8

The Holliday model is used to explain which of the following?

A) Recombination between homologous chromosomes
B) Recombination between sister chromatids
C) The creation of harlequin chromosomes
D) The action of transposons
E) None of the answers are correct

Question 9

All light chain polypeptides in an immunoglobin contain which of the following?

A) A C region
B) A V region
C) A J region
D) All of the answers are correct

Question 10

Which of the following processes accounts for the tremendous variation in antibody structure?

A) RNA editing
B) Site-specific recombination
C) Integrase activity
D) Physiological adaptation
E) Hot spots of mutation in the immunoglobin genes

Question 11

Long-terminal repeats are unique to which group of transposons?

A) Composite transposon
B) Non-viral retro element
C) Viral-like retro element
D) Replicative transposon
E) Insertion sequence

Question 12

As a researcher, you have identified a potential transposable element that contains both a transposase and resolvase enzyme. Which of the following types of transposons would you assign it to based on this information?

A) Composite transposon
B) Non-viral retro element
C) Viral-like retro element
D) Replicative transposon
E) Insertion sequence

Question 13

Which of the following domain types is unique to the heavy chain of the polypeptide?

A) V
B) D
C) J
D) C
E) None of the answers are correct

Question 14

The formation of a D-loop is associated with which of the following models of recombination?

A) Double-stranded break model
B) Holliday model
C) Single-stranded break model
D) All of the answers are correct

Question 15

RAG1 and RAG2 proteins are associated with which of the following?

A) Recombination during prophase I of meiosis
B) Nucleotide excision repair
C) Transposition
D) Integration of a bacteriophage into the host genome
E) Site-specific recombination in antibody genes

Question 16

Which of the following may account for the process of gene conversion?

A) DNA mismatch repair
B) DNA gap repair
C) Photolyase activity
D) Nucleotide excision repair
E) More than one of the above

Question 17

Which of the following genes was mobile in McClintock's experiments with mutable sites in corn?

A) Ds
B) C
C) Sh
D) Wx
E) All of the answers are correct

Question 18

The individual who first proposed the existence of transposable elements was .

A) Sturtevant
B) Morgan
C) McClintock
D) Franklin
E) Watson

Question 19

Which of the following enzymes is required for a transposon to move?

A) Integrase
B) Jumpase
C) Splicase
D) Transposase
E) None of the answers are correct

Question 20

The type of transposition in which the number of transposons increases in the genome is called .

A) Replicative transposition
B) Simple transposition
C) Site-specific transposition
D) Complex transposition

Question 21

A bacteriophage that has integrated into the bacterial chromosome is called a prophage.

Question 22

Alu is an example of a retro element in the human genome.

Question 23

In eukaryotic organisms, a single-stranded break in the DNA is required before recombination can
begin during meiosis.

Question 24

Transposase is involved with removing the transposable element from its current location.

Question 25

Transposable elements in bacteria are commonly called insertion sequences.

Question 26

Which of the following is technically not part of the genetic information of a transposable element?

A) Long-terminal repeats
B) Inverted repeats
C) Direct repeats
D) Transposase
E) All of the answers are correct

Question 27

Which of the following is an example of a transposable element?

A) Alu in Homo sapiens
B) P elements in Drosophila
C) Mu in bacteria
D) Ty in yeast
E) All of the answers are correct

Question 28

The process of recombination occurs during metaphase I of meiosis.

Question 29

Site-specific recombination occurs between homologous sequences of DNA.
FALSE

Question 30

To date, there are not any indications that transposable elements offer a selective advantage to a
species.

Question 31

A incomplete transposon contains all of the information necessary to conduct transposition.

Question 32

A composite transposon will contain only genes that are necessary for transposition.

Question 33

Transposon tagging may be used for which of the following reasons?

A) To test if a transposon is replicative
B) As a tool to clone novel genes
C) To track the movement of transposons between species
D) To inactivate transposons in a genome

Question 34

The integrase enzyme is responsible for inserting bacteriophage l into the host genome.

Question 35

Which enzyme is unique to the retro elements?

A) Reverse transcriptase
B) Reverse resolvase
C) Transposase
D) Integrase
E) Reverse integrase

Question 36

A heteroduplex is a DNA double helix that contains mismatches.

Question 37

Chromosomes that are genetically the same, but have different banding patterns using Hoechst 33258
and Giemsa stains, are called giemequin chromosomes.

Question 38

What is the selfish DNA theory?

A) A theory that explains how DNA point mutations are repaired within the genome.
B) A theory that explains how transposable elements are repaired within the genome.
C)A theory that attempts to explain the proliferation of transposable elements in a genome by comparing it to the action of a parasite.
D) None of the above

Question 39

LINEs and SINEs may be due to high levels of transposable element activity.