Deck 3: The Microbiology Laboratory

Question 1

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Agar

Answer 1

c

Question 2

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Plaque

Answer 2

b

Question 3

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Angstrom

Answer 3

e

Question 4

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Mordant

Question 5

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Slant

Question 6

The __________________lens of the microscope is closest to the specimen to be viewed.​

Question 7

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Agglutination

Question 8

A chemical additive called a _________________ is sometimes added to a laboratory stain to increase its function.​

Question 9

​Dark-field, phase-contrast, and electron are types of which of the following?

A)​Microscopes
B)​Culture media
C)​Staining techniques
D)​Specialized analyses

Question 10

​The standard unit of measure in the metric system used in the scientific community is the ______________.

Question 11

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Condenser

Question 12

A combination of nutrient agar and powdered hemoglobin is used to make which type of culture media?​

A)​Blood agar
B)​Liquid broth
C)​MacConkey
D)​Chocolate agar

Question 13

The diagnostic technique of ________________________ is used in cases of suspected rabies or syphilis.​

Question 14

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Flagella

Question 15

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Catalase

Question 16

Enriched culture media are used to grow colonies of ______________________ bacteria which have complex nutritional requirements.​

Question 17

Bacterial motility is determined by which of the following laboratory analyses?​

A)​Coagulase test
B)​Flow cytometry
C)​Hanging drop
D)​Nucleic acid hybridization

Question 18

Fluorochromes are types of which of the following?​

A)​Nutrient agar
B)​Special stains
C)​Culture media
D)​Serology assays

Question 19

Match the following terms or phrases with the correct description or definition.​
a.Ability of a lens to produce a clear image and the amount of detail produced.
b.Clear spaces on an agar plate produced by lysis of bacteria during phage-typing.
c.A solid culture medium made from red marine algae.
d.A part of the compound light microscope which focuses light onto the specimen.
e.Previously a metric unit of measure equal to 0.0000000001m or 0.1nm (nanometer).
f.Test tubes containing agar which solidified with the tubes at an angle.
g.Whip-like appendages of some species of bacteria which assist in motility.
h.Clumping together of antigen bearing microbes in the presence of specific antibodies.
i.Enzyme which causes a decomposition of hydrogen peroxide into water and oxygen.
j.A chemical additive for laboratory stains to increase their effectiveness.
​Resolution

Question 20

Which of the following microscopes is used for a 3-D view of the smallest microbes, viruses and cellular surface structures?​

A)​Compound light
B)​Fluorescence
C)​Scanning electron
D)​Transmission electron

Question 21

Prior to application of stains, specimens must be fixed to the slide to prevent washing off.​

Question 22

The wife of Walter Hesse, Robert Koch's associate, suggested the use of agar to solidify liquid culture broths.​

Question 23

The hanging drop technique for testing of bacterial motility requires use of a concave microscope slide and coverslip secured to it with petrolatum.​

Question 24

The primary stain used for spore staining is methylene blue dye.​

Question 25

The term Gram staining refers to the weight of the liquid stain used for the procedure.​

Question 26

Chocolate agar is produced using cacao powder as a rich nutrient for growing bacteria.​

Question 27

The transmission electron microscope (TEM) is used for 3-dimensional (3-D) imaging.​

Question 28

Acid-fast stains bind only to waxy coats on bacteria such as Mycobacterium tuberculosis.​

Question 29

The majority of laboratory tests/procedures are performed by unsupervised technologists.​

Question 30

Plaques appear on culture plates following performance of the catalase test demonstrating lysis of susceptible bacteria.​