Deck 4: Care and Use of the Microscope
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Deck 4: Care and Use of the Microscope
1
What is the proper way to carry a microscope?
A)Wrap one hand around the arm of the microscope.
B)Carry it with both hands supporting the stage.
C)Use one hand on the arm of the microscope, and the other hand supporting the base.
D)Carry it with one hand on each side of the base.
E)Carry it with one hand on the body tube, and the other hand under the base.
A)Wrap one hand around the arm of the microscope.
B)Carry it with both hands supporting the stage.
C)Use one hand on the arm of the microscope, and the other hand supporting the base.
D)Carry it with one hand on each side of the base.
E)Carry it with one hand on the body tube, and the other hand under the base.
C
2
When first focusing a microscope on a specimen, which objective lens should always be used first?
A)Low-power
B)Scanning
C)Oil immersion
D)High-power
E)It does not matter; any objective lens can be used first.
A)Low-power
B)Scanning
C)Oil immersion
D)High-power
E)It does not matter; any objective lens can be used first.
B
3
What is the working distance on a microscope?
A)The distance between your eye and the ocular lens
B)The distance between the condenser and the slide
C)The distance between the specimen and the slide
D)The distance between the objective lens and the slide
E)The distance between the ocular lens and the objective lens
A)The distance between your eye and the ocular lens
B)The distance between the condenser and the slide
C)The distance between the specimen and the slide
D)The distance between the objective lens and the slide
E)The distance between the ocular lens and the objective lens
D
4
All of the following practices should be followed when making a wet mount, except
A)the specimen should be covered with a drop of water.
B)the wet specimen should be covered with a coverslip.
C)the specimen can be opaque and up to 1mm in thickness.
D)the microscope should not be tilted.
E)if using the oil immersion lens, open the iris diaphragm to illuminate the specimen.
A)the specimen should be covered with a drop of water.
B)the wet specimen should be covered with a coverslip.
C)the specimen can be opaque and up to 1mm in thickness.
D)the microscope should not be tilted.
E)if using the oil immersion lens, open the iris diaphragm to illuminate the specimen.
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5
A compound microscope is one that has two ocular lenses.
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6
All of the following are true of a stereomicroscope, except
A)it can also be called a dissecting microscope.
B)it is used to view large, opaque specimens.
C)the image of the specimen is reversed and inverted.
D)it can be used to view an object 3-dimensionally.
E)it provides less magnification than a compound light microscope.
A)it can also be called a dissecting microscope.
B)it is used to view large, opaque specimens.
C)the image of the specimen is reversed and inverted.
D)it can be used to view an object 3-dimensionally.
E)it provides less magnification than a compound light microscope.
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7
The greatest magnification on the compound light microscope can be achieved by using the high power objective lens.
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8
Which part of the microscope is responsible for making small changes in focus when using the high-power objective lens?
A)Fine adjustment knob
B)Iris diaphragm lever
C)Rotating nosepiece
D)Coarse adjustment knob
E)Substage illuminator
A)Fine adjustment knob
B)Iris diaphragm lever
C)Rotating nosepiece
D)Coarse adjustment knob
E)Substage illuminator
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9
What is the typical magnification provided by the eyepiece (ocular)lens system?
A)100x
B)10x
C)4x
D)1,000x
E)0x (the eyepiece lens system does not provide any magnification)
A)100x
B)10x
C)4x
D)1,000x
E)0x (the eyepiece lens system does not provide any magnification)
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10
Which of the following parts of the microscope concentrates the light onto the microscopic slide?
A)Iris diaphragm lever
B)Substage illuminator
C)Fine adjustment knob
D)Condenser
E)Objective lens
A)Iris diaphragm lever
B)Substage illuminator
C)Fine adjustment knob
D)Condenser
E)Objective lens
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11
Why is it important to use only the fine adjustment knob when using the high-power or oil immersion lenses?
A)Using the coarse adjustment knob might crack the coverslip or slide, due to the close working distance.
B)You will not be able to focus on the specimen with the coarse adjustment knob.
C)There will not be enough light on the specimen to see it, if the coarse adjustment knob is used.
D)The condenser does not function when using the coarse adjustment knob.
E)The iris diaphragm does not function when using the coarse adjustment knob.
A)Using the coarse adjustment knob might crack the coverslip or slide, due to the close working distance.
B)You will not be able to focus on the specimen with the coarse adjustment knob.
C)There will not be enough light on the specimen to see it, if the coarse adjustment knob is used.
D)The condenser does not function when using the coarse adjustment knob.
E)The iris diaphragm does not function when using the coarse adjustment knob.
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12
If a microscopic lens is not clean, after cleaning it with lens paper or a cotton swab and lens cleaner, it should be cleaned with hydrochloric acid.
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13
When viewing a specimen on a compound light microscope, the image is right-side-up but reversed.
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14
The magnification of a specimen viewed with a 10x eyepiece and a 40x high-power objective lens is ________.
A)50x
B)40x
C)10x
D)400x
E)500x
A)50x
B)40x
C)10x
D)400x
E)500x
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15
A parfocal microscope is one that keeps the specimen in focus (or very close to it)when a higher-power objective lens is rotated into position, after focusing with a lower-power objective.
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