Genetics: Analysis and Principles 4th Edition by Robert Brooker

Exercise 4

In bacteria, researchers have isolated strains that carry mutations within tRNA genes. These mutations can change the sequence of the anticodon. For example, a normal tRNA Trp gene would encode a tRNA with the anticodon 3 -ACC-5 . A mutation could change this sequence tO₃ -CCC-5 . When this mutation occurs, the tRNA still carries a tryptophan at its 3 acceptor stem, even though the anticodon sequence has been altered.
A. How would this mutation affect the synthesis of polypeptides within the bacterium
B. What does this mutation tell you about the recognition between tryptophanyl-tRNA synthetase and tRNA Trp Does the enzyme primarily recognize the anticodon or not