Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 33

Can you devise an experimental strategy to get P1 phage to transduce the entire genome from one strain of bacterium to another strain (Note: The general features of phage lambda's reproductive cycle are described in Chapter 17.) Phage has a genome size of 48,502 nucleotides (about 1% of the size of the E. coli chromosome) and can follow the lytic or lysogenic reproductive cycle. Growth of E. coli on minimal growth medium favors the lysogenic reproductive cycle, whereas growth on a rich medium and/or under UV light promotes the lytic cycle.