Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 40

Take a look at solved problem S1. Let's suppose a male is heterozygous for two polymorphic sequence-tagged sites. STS-1 exists in two sizes: 211 bp and 289 bp. STS-2 also exists in two sizes: 115 bp and 422 bp. A sample of sperm was collected from this man, and individual sperm were placed into 30 separate tubes. Into each of the 30 tubes were added the primers that amplify STS-1 and STS-2, and then the samples were subjected to PCR. The following results were obtained:

a. What is the arrangement of these STSs in this individual
B. What is the linkage distance between STS-1 and STS-2
C. Could this approach of analyzing a population of sperm be applied to RFLPs