Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 14

A human gene, which we will call gene X , is located on chromosome 11 and is found as a normal allele and a recessive disease-causing allele. The location of gene X has been approximated on the map shown here that contains four STSs, labeled STS-1, STS-2, STS-3, and STS-4.
a. Explain the general strategy of positional cloning
b. If you applied the approach of positional cloning to clone gene X , where would you begin As you progressed in your cloning efforts, how would you know if you were walking toward or away from gene X
C. How would you know you had reached gene X (Keep in mind that gene X exists as a normal allele and a disease-causing allele.)