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iGenetics 3rd Edition by Peter Russell
Edition 3ISBN: 978-0321569769iGenetics 3rd Edition by Peter Russell
Edition 3ISBN: 978-0321569769 Exercise 3
A spaceship lands on Earth, bringing with it a sample of extraterrestrial bacteria. You are assigned the task of determining the mechanism of DNA replication in this organism.
You grow the bacteria in an unlabeled medium for several generations and then grow it in the presence of for exactly one generation. You extract the DNA and subject it to CsCl centrifugation. The banding pattern you find is as follows:
It appears to you that this pattern is evidence that DNA replicates in the semiconservative manner, but you are wrong. Why? What other experiment could you perform (using the same sample and technique of CsCl centrifugation) that would further distinguish between semiconservative and dispersive modes of replication?
You grow the bacteria in an unlabeled medium for several generations and then grow it in the presence of for exactly one generation. You extract the DNA and subject it to CsCl centrifugation. The banding pattern you find is as follows:
It appears to you that this pattern is evidence that DNA replicates in the semiconservative manner, but you are wrong. Why? What other experiment could you perform (using the same sample and technique of CsCl centrifugation) that would further distinguish between semiconservative and dispersive modes of replication?
Explanation
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Even if the deoxyribonucleic acid (DNA) ...
iGenetics 3rd Edition by Peter Russell
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