Essential Genetics 6th Edition by Daniel Hartl

Exercise 14

Shown in the accompanying gel are seven DNA fragments, designated A-G, present along the X chromosome of the laboratory rat Rattus norvegicus. Each fragment was amplified by means of the polymerase chain reaction (PCR), as described in Chapter 6, using a unique pair of primers. The seven fragments are present in close proximity to each other along the X chromosome. Five small deletions in the X chromosome, designated ? 1 -? 5 , present in different strains, delete at least one of the templates for amplification. The gel shows the amplification products obtained from genomic DNA from males carrying each of the five deletions. Deduce the linear order of the amplified fragments A2G present in the wildtype (undeleted) X chromosome.