Essential Genetics 6th Edition by Daniel Hartl

Exercise 3

A mutant allele is found to express the wildtype gene product but at only about 20 percent of the wildtype level. The mutation is traced to an intron whose size has increased by 3.1 kb because of the presence of a DNA fragment with the restriction map shown here. The symbols A, B, C, D, E, H, K, P, S, and X represent cleavage sites for the restriction enzymes Alu I, Bam HI, Cla I, Dde I, Eco RI, Hind III, Kpn I, Pst I, Sac I, and Xho II, respectively. Does the restriction map of the insertion give any clues to what it is?