Modern Experimental Biochemistry 3rd Edition by Rodney Boyer

Exercise 6

Assume that you use the standard curve produced in Study Problem to measure the concentration of an unknown protein. The A 595 for 1.0 mL of the unknown was 0.52. Prepare a standard curve from the data in Problem and estimate the concentration of unknown protein in the sample in ? g/mL.
Below is a table prepared by a biochemistry student to construct a standard curve for protein analysis. The Bradford assay was used with bovine serum albumin (BSA, 0.1 mg/mL) as standard protein. Complete the table by filling in the weight of BSA in each tube and the approximate A 595 that will be obtained for each tube. Assume the procedure was con ducted correctly.