
Introduction to Biotechnology 3rd Edition by William Thieman ,Michael Palladino
Edition 3ISBN: 978-0321766113
Introduction to Biotechnology 3rd Edition by William Thieman ,Michael Palladino
Edition 3ISBN: 978-0321766113 Exercise 7
Consider the following sequence of DNA: 5'-TTTATGGG TTGGCCCGGGTCATGATT- 3' 3'-AAATCCCAACCGGGCCCAGT ACTAA- 5'
a. Transcribe each of these sequences into mRNA. Which DNA sequence (top or bottom strand) produces a functional mRNA containing a start codon What is the amino acid sequence of the polypeptide produced from this mRNA
b. For the DNA strand producing a functional mRNA, number each base from left to right with the first base numbered "1." Insert a "T" between bases 10 and 11, representing a base insertion mutation. Transcribe an mRNA from this new strand and translate it into a protein. Compare the amino acid sequence of this protein to the one translated in (a). What happened Explain.
a. Transcribe each of these sequences into mRNA. Which DNA sequence (top or bottom strand) produces a functional mRNA containing a start codon What is the amino acid sequence of the polypeptide produced from this mRNA
b. For the DNA strand producing a functional mRNA, number each base from left to right with the first base numbered "1." Insert a "T" between bases 10 and 11, representing a base insertion mutation. Transcribe an mRNA from this new strand and translate it into a protein. Compare the amino acid sequence of this protein to the one translated in (a). What happened Explain.
Explanation
a) The sequence of the DNA given is
Th...
Introduction to Biotechnology 3rd Edition by William Thieman ,Michael Palladino
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