Introduction to Biotechnology 3rd Edition by William Thieman ,Michael Palladino

Exercise 3

Your lab just determined the sequence of a rat gene thought to be involved in controlling the fertilizing ability of rat sperm. You believe a similar gene may control fertility in human males. Briefly describe how you could use what you know about this rat gene combined with PCR to clone the complementary human gene. Be sure to explain your experimental approach and the necessary lab materials. Also explain in detail any procedures necessary to confirm that you have a human gene that corresponds to your rat gene.