Campbell Biology 11th Edition by Lisa Urry,Michael Cain,Steven Wasserman,Peter Minorsky,Jane Reece

Exercise 14

Draw I T You are cloning an aardvark gene, using a bacterial plasmid as a vector. The green diagram shows the plasmid, which contains the restriction site for the enzyme used in Figure 20.6. Above the plasmid is a segment of linear aardvark DNA that was synthesized using PCR. Diagram your cloning procedure, showing what would happen to these two molecules during each step. Use one color for the aardvark DNA and its bases and another color for those of the plasmid. Label each step and all 5 and 3 ends.