Microbiology 9th Edition by Laura Black, Jacquelyn Black

Exercise 28

Katy started the primary crystal violet of her Gram stain on her "unknown" culture, but ran out of time. She left the slide in her drawer, with plans to complete the washes and staining next week. What has she overlooked that may prevent her from obtaining proper results? If Katy were to initiate a new Gram stain on a smear she made from last week's bacterial culture, would she get good, reliable results? Why or why not?