Deck 20: Vibrio, Aeromonas, and Campylobacter Species

Question 1

What two biochemical tests help distinguish Aeromonas spp. from other enterics?

A) Oxidase and indole
B) Oxidase and Simmon's citrate
C) Simmon's citrate and indole
D) Voges-Proskauer (VP) and indole

Answer 1

A

Question 2

Which of the following is a curved, gram-negative rod that grows on thiosulfate citrate bile salts sucrose (TCBS) agar?

A) Vibrio
B) Campylobacter
C) Clostridium
D) Fusobacterium

Answer 2

A

Question 3

All of the following are biochemical characteristics of Vibrio spp., except

A) ferments glucose.
B) reduces nitrate to nitrite.
C) halophilic.
D) oxidase negative.

Answer 3

D

Question 4

Most Vibrio sp. are generally halophilic. However, which one can also grow in an environment with 0% sodium chloride?

A) V. cholerae
B) A. parahaemolyticus
C) V. alginoltyticus
D) V. vulnificus

Question 5

All of the following characteristics describe the genus Plesiomonas, except

A) ferments glucose.
B) motile.
C) oxidase negative.
D) facultative anaerobes.

Question 6

What is the name of the organism that is responsible for "summer diarrhea" in Japan?

A) Vibrio cholerae
B) V. vulnificus
C) V. parahaemolyticus
D) V. trota

Question 7

What species of Aeromonas is the most frequently associated with gastrointestinal infections?

A) A. hydrophila
B) A. caviae
C) A. veronii
D) A. sobria

Question 8

Most infections caused by Vibrio cholerae are associated with

A) arthropod bites.
B) ingestion of undercooked beef and pork.
C) ingestion of undercooked seafood.
D) swimming in ocean water.

Question 9

What disease manifests in acute cases as a severe gastroenteritis, accompanied by vomiting followed by diarrheic stools that are described as rice water and occur 10 to 30 times a day?

A) Cholera
B) Typhoid
C) Dysentery
D) Food poisoning

Question 10

What do aeromonad colonies look like on cefsulodin-irgasan-novobiocin (CIN) agar?

A) Purple colonies
B) Clear colonies
C) Green colonies
D) Pink colonies

Question 11

What environment is Aeromonas spp. most commonly found in?

A) Arthropods
B) Wild animals
C) Ocean water
D) Fresh water

Question 12

Aeromonads are generally susceptible to all these antimicrobial agents, except

A) trimethoprim-sulfamethoxazole.
B) ampicillin.
C) aminoglycosides.
D) quinolones.

Question 13

Aeromonads have been implicated in all of the following diseases, except

A) osteomyelitis.
B) meningitis.
C) pneumonia.
D) otitis.

Question 14

How is cholera treated?

A) Tetracycline
B) Copious amounts of intravenous fluids
C) Aminoglycosides with a large amount of intravenous fluids
D) Rest and cold showers

Question 15

When collecting specimens that may contain Campylobacter spp. bacteria, what transport medium should they be placed in, if a delay in transport is possible?

A) Buffered glycerol-saline
B) Regan-Lewis
C) Aimes
D) Cary-Blair

Question 16

What is the name of the organism that causes the disease cholera?

A) Vibrio furnissii
B) V. cholerae
C) V. vulnificus
D) V. parahaemolyticus

Question 17

What is the name of the organism that is strongly associated with gastric, peptic, and duodenal ulcers, as well as gastrointestinal carcinoma?

A) Campylobacter jejuni
B) Campylobacter curvus
C) Helicobacter pylori
D) Helicobacter rectus

Question 18

Which of the following Vibrio species is most likely to be recovered from extraintestinal tract infections,e.g., septicemia?

A) Vibrio alginolyticus
B) V. parahaemolyticus
C) V. cholerae
D) V. vulnificus

Question 19

What organism may play a role in Guillain-Barré syndrome (GBS)?

A) Helicobacter
B) Aeromonas
C) Campylobacter
D) Plesiomonas

Question 20

A patient is brought to the emergency department with a severe case of gastroenteritis. The patient experienced gastrointestinal upset after eating raw oysters. What organism can be the culprit for this patient's condition?

A) Vibrio trota
B) V. parahaemolyticus
C) V. cholerae
D) V. vulnificus

Question 21

How is Helicobacter pylori presumptively identified?

A) A culture of a gastric biopsy
B) A culture of gastric scrapings
C) A rapid oxidase test done on gastric scrapings
D) A rapid urease test done on a gastric biopsy

Question 22

What type of an atmosphere do campylobacters require for growth?

A) Microaerophilic and capnophilic
B) Anaerobic and moist
C) Capnophilic and halophilic
D) Microaerophilic and high nitrogen content

Question 23

What organism has a microscopic morphology of tiny, curved, gram-negative rods, with S-shapes or seagull-wing shapes on Gram stain?

A) Helicobacter
B) Campylobacter
C) Haemophilus
D) Acinetobacter

Question 24

What is the drug of choice for treating intestinal campylobacteriosis?

A) Erythromycin
B) Gentamicin
C) Vancomycin
D) Imipenem

Question 25

What temperature is an optimal growth temperature for Campylobacter jejuni?

A) 35° C
B) 42° C
C) 25° C
D) 60° C

Question 26

A Campy blood agar plate incubated at 42° C from a stool exhibits colonies that are nonhemolytic, moist, "runny looking," and spreading. The Gram stain shows tiny gram-negative rods with some S-shapes and seagull-wing shapes. What is growing on the plate?

A) Aeromonas
B) Helicobacter
C) Campylobacter
D) Acinetobacter

Question 27

CAMPY-BAP (blood agar plate) contains

A) Brucella agar base, 10% sheep red blood cells, vancomycin, trimethoprim, polymyxin B, amphotericin B, and cephalothin.
B) Columbia agar base, 5% sheep red blood cells, erythromycin, spectinomycin, Diflucan, and ampicillin.
C) Brucella agar base, 5% sheep red blood cells, Zithromax, sulfamethoxazole, nystatin, amphotericin B, and imipenem.
D) Columbia agar base, glycerol, L-cysteine, hemin, streptomycin, amphotericin B, and gentamicin.