Deck 39: Automation in Hematology

Question 1

Which of the following is a reported parameter for the ADVIA 120 but not for the LH 750 instrument?

A) RDW-CV
B) Retic%
C) CHCM
D) MCVr

Answer 1

CHCM

Question 2

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

A) Advia 120
B) Coulter LH series
C) Abbott Sapphire
D) Sysmex XE-2100

Answer 2

Coulter LH series

Question 3

Which of the following analyzers uses radio frequency to detect and differentiate leukocytes?

A) Bayer Advia 2120
B) Abbott Sapphire
C) Sysmex XE-2100
D) Coulter Gen-S

Answer 3

Sysmex XE-2100

Question 4

Hydrodynamic focusing is used in automated cell-counting instruments to:

A) Prevent coincidence passage through the detection aperture
B) Increase the visibility of the cells passing through the aperture
C) Allow for maximal light absorbance through the cells passing through the aperture
D) Ensure adequate uptake of fluorescent staining by the cells

Question 5

What cellular characteristic is evaluated by radio frequency?

A) Size
B) Density
C) Shape
D) Complexity

Question 6

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

A) Coulter Gen-S
B) Sysmex XE-2100
C) Abbott Cell Dyn Sapphire
D) Coulter LH 780

Question 7

Cell volume and cell number are required elements for which of the following?

A) WBC scatterplots
B) WBC cytograms
C) RBC histograms
D) RBC scattergrams

Question 8

Which analyzer does not use cyanide-free detection of hemoglobin?

A) Advia 120
B) Advia 2120
C) Abbott Cell-Dyn Sapphire
D) Sysmex XE-2100

Question 9

The Sysmex XE-2100 instrument printout reports a middle fluorescent ratio of 7.0 and a high fluorescent ratio of 1.3. What is the immature reticulocyte fraction?

A) 9.1
B) 91.7
C) 8.3
D) 5.7

Question 10

Which parameter does the Sysmex XE-2100 instrument determine by using light-scattering and fluorescence intensity characteristics?

A) Platelet count
B) Erythrocyte count
C) Absolute basophil count
D) Absolute lymphocyte count

Question 11

Which of the following instruments does not rely on impedance to enumerate cells?

A) Advia 120
B) XE-2100
C) Sapphire
D) Coulter

Question 12

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

A) 0 degrees
B) 7 degrees
C) 90 degrees
D) 90 degrees depolarized

Question 13

Which parameter does the Coulter LH 750 instrument directly measure from a histogram?

A) Platelet count
B) MCH
C) Leukocyte count
D) Hct

Question 14

In the reticulocyte determination, using the Sysmex XE-2100, the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells. What does the oxazine bind to within the cells?

A) Residual DNA
B) Hemoglobin
C) Residual RNA
D) Mitochondria

Question 15

The principle that relies on detecting resistance as cells pass through a photo-detector and is used by most of the major hematology analyzers is:

A) Spectrophotometry
B) Impedance
C) Radio frequency
D) MAPSS technology

Question 16

Which of the following parameters are calculated on the Coulter LH?

A) Hemoglobin, MCH, MCHC
B) Hematocrit, MCH, MCHC
C) Absolute neutrophil count, RBC count, platelet count
D) RBC count, hematocrit, MCH

Question 17

Regarding the CELL-DYN Sapphire, which parameter is determined by the impedance principle?

A) Hemoglobin
B) Nucleated erythrocyte count
C) Platelet count
D) Total leukocyte count

Question 18

Which analyzer measures platelets in the same channel as reticulocytes?

A) Coulter Gen-S
B) Sysmex XE-2100
C) Advia 2120
D) Trinity Biotech AMAX Destiny Plus

Question 19

Which instrument does not use fluorescence intensity in its determination of reticulocytes?

A) LH 750
B) XE-2100
C) ADVIA 120
D) CELL-DYN Sapphire

Question 20

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

A) New methylene blue
B) Propidium iodide
C) Polymethine
D) Oxazine

Question 21

When using the CELL-DYN Sapphire to perform CBCs, a laboratory professional can choose to run the ImmunoPlt assay if:

A) Leukocyte count is decreased
B) Erythrocyte fragments are detected
C) Nucleated erythrocytes are observed
D) Erythrocyte count is increased

Question 22

Which technologies are used to generate the LH 750's WBC scatterplot?

A) Impedance, light scatter, and conductivity
B) Fluorescence intensity and radio frequency
C) Absorbance and conductivity
D) Light scatter, absorbance, and radio frequency

Question 23

Compare and contrast the methods of detection for the following analyzers:
a. Sysmex XE-5000
b. Abbott Cell-Dyn Sapphire
c. Coulter DxH 800
d. Advia 2120

Question 24

Which instrument utilizes contour gating to classify different leukocyte cell types?

A) ADVIA 120
B) XE-2100
C) LH 750
D) CELL-DYN Sapphire

Question 25

The immature platelet fraction (IPF) is a parameter derived from the Sysmex XE-2100's RET scattergram. An increased IPF is associated with:

A) Aplastic anemia
B) Pernicious anemia
C) Acute lymphocytic leukemia
D) Immune thrombocytopenic purpura

Question 26

What is the principle of impedance? Name at least two instruments that employ this method for blood cell counting.

Question 27

Explain the purpose of the following used in the standard hematologic cell-counting detection methods:
a. VCS flow cell
b. Photodetector
c. Sheath fluid
d. Direct current detection block