Question 1

DNA fragments separated on an agarose gel can be visualized after staining with ____&#10;A)ethidium bromide&#10;B)bromophenol blue&#10;C)acetocarmine&#10;D)aniline blue

Accepted Answer

Ethidium bromide is a commonly used stain for visualizing DNA fragments on an agarose gel under UV light. It intercalates between the DNA bases, allowing the fragments to be seen.

Question 2

A gene whose expression helps to identify transformed cells is known as ____&#10;A)Plasmid&#10;B)Selectable marker&#10;C)Structural gene&#10;D)vector

Accepted Answer

Selectable markers help in identifying or selecting the cells that have been successfully transformed with a new gene.

Question 3

A single strand of nucleic acid tagged with a radioactive molecule is called&#10;A)Plasmid&#10;B)Probe&#10;C)selectable marker&#10;D)Vector

Accepted Answer

A probe is a single-stranded sequence of DNA or RNA used in molecular biology to detect the presence of complementary sequences by hybridization. Probes are often tagged with radioactive molecules to allow for their detection in various assays.

Question 4

There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?&#10;A)Coli&#10;B)Colon&#10;C)Cofactor&#10;D)None of the above

Accepted Answer

The 'co' in Eco RI stands for 'coli', as in Escherichia coli, the bacterium from which this restriction enzyme was first isolated.

Question 5

Agarose extracted from sea weeds finds use in ____&#10;A)Spectrophotometry&#10;B)Gel electrophoresis&#10;C)PCR&#10;D)tissue culture

Accepted Answer

Agarose, extracted from seaweed, is primarily used in gel electrophoresis for the separation of nucleic acids (DNA or RNA) based on their size. This makes it a fundamental tool in molecular biology for analyzing and purifying nucleic acids.

Question 6

Animal biotechnology involves&#10;A)production of valuable products in animals using rDNA technology&#10;B)rapid multiplication of animals of desired genotypes&#10;C)alteration of genes to make it more desirable&#10;D)all of these

Accepted Answer

Animal biotechnology encompasses the use of recombinant DNA technology to produce valuable products in animals, the rapid multiplication of animals with desired genotypes, and the alteration of genes to enhance desirable traits.

Question 7

Animal cell cultures are used widely for the production of&#10;A)insulin&#10;B)somatostatin&#10;C)mabs&#10;D)thyroxine

Accepted Answer

Monoclonal antibodies (mabs) are produced using animal cell cultures, as they require complex post-translational modifications that can only be performed by eukaryotic cells.

Question 8

The first vaccine developed from animal cell culture was&#10;A)Hepatitis B vaccine&#10;B)Influenza vaccine&#10;C)Small pox vaccine&#10;D)Polio vaccine

Accepted Answer

The first vaccine developed from animal cell culture was the Polio vaccine. This method allowed for the large-scale production of vaccines and was a significant advancement in vaccine technology.

Question 9

Which of the following are commonly produced in animal cell cultures&#10;A)Interferon&#10;B)mab&#10;C)vaccines&#10;D)all of these

Accepted Answer

Animal cell cultures are widely used for the production of interferons, monoclonal antibodies (mAb), and vaccines, making all of these options correct.

Question 10

The cell line used for the production of polio vaccine was&#10;A)Primate kidney cell line&#10;B)CHO cell line&#10;C)Dog kidney cell line&#10;D)mouse fibroblast cell line

Accepted Answer

The polio vaccine was historically produced using primary cultures from primate kidney cells. These cells were used because they could support the growth of the poliovirus, allowing for the production of vaccine doses.

Question 11

Recombinant proteins are&#10;A)proteins synthesized in animals&#10;B)proteins synthesized by transgene in host cell by rDNA technology&#10;C)proteins synthesised in cells that are produced by protoplast fusion&#10;D)proteins synthesized in mutated cell lines

Accepted Answer

The answer of Recombinant proteins are&#10;A)proteins synthesized in animals&#10;B)proteins synthesized...

Question 12

Interferons are&#10;A)anti bacterial proteins&#10;B)anti-viral proteins&#10;C)bacteriostatic proteins&#10;D)all of these

Accepted Answer

The answer of Interferons are&#10;A)anti bacterial proteins&#10;B)anti-viral proteins&#10;C)bacteriostatic proteins&#10;D)all of...

Question 13

The virus commonly used to infect cell cultures for the production of interferon is&#10;A)Corona virus&#10;B)Sendai virus&#10;C)Polio virus&#10;D)Small pox virus

Accepted Answer

The answer of The virus commonly used to infect cell...

Question 14

Hybrid antibodies are&#10;A)antibodies produced in cell cultures&#10;B)antibodies designed using rDNA technology produced in cell cultures&#10;C)antibodies produced in invivo&#10;D)both a and b

Accepted Answer

The answer of Hybrid antibodies are&#10;A)antibodies produced in cell cultures&#10;B)antibodies...

Question 15

The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is&#10;A)protoplast fusion and embryo transfer&#10;B)hybrid selection and embryo transfer&#10;C)in vitro fertilization and embryo transfer&#10;D)all of these

Accepted Answer

The answer of The technique used in animal biotechnology for...

Question 16

The production of complete animals from somatic cells of an animal is called&#10;A)gene cloning&#10;B)animal cloning&#10;C)cell cloning&#10;D)all of these

Accepted Answer

The answer of The production of complete animals from somatic...

Question 17

In humans, the babies produced by in vitro fertilization and embryo transfer was popularly called as&#10;A)invitro babies&#10;B)test tube babies&#10;C)invitro-invivo babies&#10;D)all of these

Accepted Answer

The answer of In humans, the babies produced by in...

Deck 2: Biotechnology and Animal Cell Culture