Deck 42: Basic Microbiology

Question 1

Which of the following methods is used for maintaining an anaerobic condition?

A)Incubator
B)Gas pack jar  
C)Petri dish
D)Safety hood

Answer 1

Gas pack jar  

Question 2

Which of the following best describes how a bacterium is determined to be Gram positive or negative?

A)Nature of the cytoplasm
B)Ability to retain or lose color  
C)Presence of ribosomes
D)Absence of cytoplasm

Answer 2

Ability to retain or lose color  

Question 3

Which of the following best describes placing a sample of body secretions on a special medium to allow bacteria to grow?

A)Sensitivity test
B)Culture  
C)Holding media
D)Mordant

Answer 3

Culture  

Question 4

Which of the following is the nuclear material in all cells?

A)Ribosomes
B)Cytoplasm  
C)DNA
D)RNA

Question 5

Which of the following best describes the use of a culturette tube?

A)Transporting stool samples
B)Keeping the swab moist  
C)Transporting blood specimens
D)All the above

Question 6

Which of the following is NOT part of a quality control program in microbiology?

A)Monitor all equipment with temperature controls daily. 
B)Do not use media past the shelf life. 
C)Update the laboratory manual daily. 
D)Make the SDS available to reference.

Question 7

Which of the following is the type of stain that is often referred to as a specific stain and that allows for microscopic examination of acid-fast organisms?

A)Gram
B)Acid-fast  
C)Simple
D)None of the above

Question 8

Mycology is the study of

A)Protozoa
B)Algae  
C)Bacteria
D)Fungi

Question 9

Which of the following is NOT an appropriate action when handling microbiology specimens?

A)Look for leaks on the outside of the transporting container. 
B)Wear gloves. 
C)Use the appropriate container when sending specimens to an outside lab. 
D)Handle the specimen as if it is not contaminated.

Question 10

Which of the following best describes when a safety hood is mandatory in laboratory use?

A)Performing a culture with a potential of aerosol  
B)Examining a stool specimen  
C)Working with a foul-smelling specimen  
D)Performing an anaerobic culture

Question 11

Media that that contains substances that alter the appearance of some organisms and not others is called:

A)Differential media
B)Basic media  
C)Selective media
D)Enriched media

Question 12

The correct specimen and diagnostic test used to identify ova and parasites is

A)Urine and a culture and sensitivity
B)Vaginal and culture  
C)Stool and culture
D)None of the above

Question 13

Which of the following should be used when obtaining a nasopharyngeal collection for a culture?

A)Sterile tongue depressor
B)Sterile applicator stick  
C)Special swab on a thin wire
D)None of the above

Question 14

Which of the following applies to the collection of specimens?

A)Lab assistants do not need to wear gloves. 
B)Wear gloves only when needed. 
C)Occasionally follow instructions in the lab manual. 
D)Must strictly adhere to standard precautions.

Question 15

Which of the following best describes the results of a Gram stain?

A)All bacteria will turn yellow. 
B)Gram negative bacteria will turn purple. 
C)Gram positive bacteria will turn purple. 
D)Gram positive bacteria will not have any color.

Question 16

A wet mount is used to:

A)Stain bacteria
B)Suspend the organism in a liquid to study  
C)Examine organisms under a microscope
D)Both b and c

Question 17

Which of the following type of specimen is obtained when a lumbar puncture is performed by the physician?

A)Sputum
B)Stool  
C)Mucous
D)Cerebrospinal fluid

Question 18

Which of the following is NOT given out as patient education to relieve discomfort and help patients toward better health?

A)Dress comfortably according to the weather. 
B)Avoid tobacco. 
C)Cough or sneeze into a tissue. 
D)Decrease water intake.

Question 19

Which of the following terms best describes infections acquired in hospitals?

A)Floral
B)Bacterial  
C)Serratia
D)Nosocomial

Question 20

Which of the following is used for a stab culture?

A)Inoculating loop
B)Inoculating needle  
C)Incinerator
D)Bunsen burner

Question 21

What is the organism that causes strep throat?

A)Group A, alpha-hemolytic streptococcus
B)Group A, beta-hemolytic streptococcus  
C)Group B, alpha-hemolytic streptococcus
D)Group B, beta-hemolytic streptococcus

Question 22

Which of the following is true regarding culture media?

A)Media can be solid, liquid, or gas.
B)The agar is considered liquid form. 
C)Agar  contains nutrients in a prepared petri dish
D)Semisolid is made by adding more agar.

Question 23

From which of the following is the organism Neisseria meningitidis obtained?

A)Genitalia
B)Cerebrospinal fluid  
C)Sputum
D)Urine

Question 24

Giardiasis Lamblia is often contracted by which of the following?

A)Mountain hikers
B)Children  
C)Sanitation workers
D)Athletes

Question 25

When a patient has a bacterial infection that is not responding to the antibiotic, what test might the physician order?

A)Differential stain
B)C & S  
C)KOH
D)All the above

Question 26

Foodborne illnesses are often caused by which of the following?

A)Raw or uncooked food
B)Contaiminated food handlers  
C)Both A & B
D)Fish from polluted waters

Question 27

After the agar plate has been inoculated and properly labeled, what must you do with the plate?

A)Nothing, agar plates require nothing special. 
B)Place it back with the other agar plates in the original package. 
C)Leave it right-side-up, and place in the proper environment for growth. 
D)Turn it upside down, and place in the proper environment for growth.

Question 28

How can a fungus infection be diagnosed?

A)Use a Woody lamp.
B)Use a Wood's lamp. 
C)Use a Wooden lamp.
D)Use a Wood's lantern.

Question 29

Which of the following best describes the use of the colony streaking technique?

A)Dilute bacteria
B)Grow yeast  
C)Plate urine cultures
D)Grow Shigella

Question 30

Which of the following bacteria are purple rods when the staining process is complete?

A)Gram-negative bacilli
B)Gram-positive bacilli  
C)Gram-positive cocci
D)Gram-negative spirilla

Question 31

Which of the following is the KOH used for testing?

A)Gram positive bacteria
B)Acid-fast bacteria  
C)Fungi and spores
D)Stool specimens

Question 32

Which of the following is an organism requiring special media and an atmosphere of reduced oxygen and increased carbon dioxide?

A)Streptococcus
B)Neisseria gonorrhoeae  
C)Staphylococcus
D)Escherichia coli

Question 33

Which of the following is NOT included in inoculating techniques?

A)Loop
B)Lawn streak  
C)Colony count
D)Random streak

Question 34

When a parasite test comes back negative for worms, how many confirmatory tests should be performed?

A)5
B)several  
C)7
D)8

Question 35

Which of the following is the next step when there is more than one pathogen growing in a bacterial culture?

A)Use a dissecting microscope. 
B)Wait an additional 36 hours to identify the predominant organism. 
C)Obtain another specimen. 
D)Set up a subculture and separate bacteria to another media.

Question 36

Which of the following is the type of media classification that supports the growth of one type of organism while inhibiting the growth of another?

A)Basic
B)Differential  
C)Selective
D)Enriched

Question 37

Which of the following is true when collecting stool for O & P identification?

A)Do not contaminate the stool specimen with urine. 
B)Use special vials. 
C)Collect the stool in wide-mouth containers. 
D)All the above.

Question 38

If a spill should occur in the lab, which of the the following agents is used for cleanup:

A)2% phenol
B)10% bleach  
C)10% phenol
D)3% bleach

Question 39

Which of the following bacteria are round in shape and can occur in clusters or pairs?

A)Spirilla
B)Bacilli  
C)Cocci
D)None of the above

Question 40

When using KOH on a wet mount, what does the KOH do?

A)Stains the organism
B)Destroys the fungus  
C)Clears debris
D)Makes objects look brighter

Question 41

Trichomonas vaginalis is a parasite that is found how many more times in women than men?

A)2
B)6  
C)5
D)4