The appropriate sequence in the biosynthesis of proline from glutamate is: 1.NADPH reduction and release of P_i
2) Dehydration to 1-pyrroline-5-carboxylate
3) NADPH reduction of Schiff base
4) Phosphorylation to glutamate-5-phosphate

Question

Quizplus · Accepted Answer

The first step in proline biosynthesis is the phosphorylation of glutamate to form glutamate-5-phosphate (step 4). This is followed by the dehydration of glutamate-5-phosphate to form 1-pyrroline-5-carboxylate (step 2). The third step involves the NADPH reduction of a Schiff base intermediate, which ultimately leads to the release of pyrroline-5-carboxylate (step 1). Finally, the pyrroline-5-carboxylate is reduced by NADPH to form proline (step 3). Therefore, the appropriate sequence is 4, 1, 2, 3, giving option B as the best choice.

The Appropriate Sequence in the Biosynthesis of Proline from Glutamate