Which of the Following Effects Is Possible If LEF1 Were $\alpha$

Which of the following effects is possible if LEF1 were omitted from an in vitro transcription assay that you were conducting to look at the expression of the TCR $\alpha$ gene?

A) no transcription
B) low levels of transcription
C) lack of bending in the promoter DNA
D) rapid initiation of transcription
E) no transcription,low levels of transcription,and lack of bending in the promoter DNA are all possible outcomes.